Clinical feature and genetic analysis of a patient with Idiopathic hypogonadotropic hypogonadism due to a novel variant of CHD7 gene / 中华医学遗传学杂志

OBJECTIVE@#To explore the clinical feature and genetic etiology of a patient with normosmic idiopathic hypogonadotropic hypogonadism (nIHH) due to variant of CHD7 gene.@*METHODS@#A patient who had presented at Anhui Provincial Children's Hospital in October 2022 was selected as the study subject. Clinical data of the patient was collected. The patient and his parents were subjected to trio-whole exome sequencing. Candidate variant was verified by Sanger sequencing and bioinformatic analysis.@*RESULTS@#The patient had featured delayed development of secondary sexual characteristics but normal olfactory function. Genetic testing revealed that he has harbored a c.3052C>T (p.Pro1018Ser) missense variant of the CHD7 gene, for which both of his parents were of the wild type. The variant has not been recorded in the PubMed and HGMD databases. Analysis of amino acid sequences suggested that the variant site is highly conserved, and the variant may affect the stability of protein structure. Based on the guidelines from the American College of Medical Genetics and Genomics, the c.3032C>T variant was classified as a likely pathogenic (PS2+PM2_Supporting+PP2+PP3+PP4).@*CONCLUSION@#The delayed development of secondary sexual characteristics of the patient may be attributed to the c.3052C>T (p.Pro1018Ser) variant of the CHD7 gene. Above finding has expanded the variation spectrum of the CHD7 gene.

Topological Structure and Biomechanics of Three-Dimensional Printed Height Increasing Insoles for Leg Length Discrepancy / 医用生物力学

Objective To study topological structure of a new type of three-dimensional (3D) printed height increasing insoles for leg length discrepancy (LLD) and its effect on biomechanics of lower limbs. Methods Topological structure for middle and rear part of the insole was optimized by solid isotropic microstructures with penalization (SIMP), the force was loaded and the boundary conditions were set according to force area of the insole, and the height increasing insole with thermoplastic polyurethanes (TPU) materials was printed by selected laser sintering (SLS). The insoles were used in 9 patients with LLD, visual analogue scale (VAS) and Maryland foot function scores were used to compare pain and foot function changes of patients before and after using the insole, and the 3D gait analysis system was used to compare spatiotemporal parameters and vertical ground reaction force (vGRF) of both lower limbs. Result sAfter the patient wore 3D printed insole, VAS scores decreased, Maryland foot function scores increased, vGRF of both lower limbs decreased, and the difference of cadence, stance phase and swing phase in both lower limbs decreased. Conclusions The 3D printed height increasing insole after topology optimization can improve coordination of lower limb movement, reduce ground impact, relieve pain and improve foot function, thus providing an effective personalized orthopedic plan for LLD treatment in clinic.

Clinical characteristics and genetic analysis of an ethnic Han Chinese child with Keppen-Lubinsky syndrome due to a de novo KCNJ6 mutation / 中华医学遗传学杂志

OBJECTIVE@#To investigate the clinical characteristics and genetic basis for a child with Keppen-Lubinsky syndrome (KPLBS).@*METHODS@#Trio-whole exome sequencing (Trio-WES) was carried out for the proband and her parents. Candidate variant was verified by Sanger sequencing and bioinformatic analysis.@*RESULTS@#The child has featured peculiar facies including large eyes, alar hypoplasia, microretrognathia, premature aging appearance in addition with growth delay and mental retardation. Trio-WES has identified that she has carried a de novo variant of the KCNJ6 gene, namely c.460G>C (p.Gly154Arg). The variant has not been recorded in the database. Prediction of protein structure indicated that the variant may affect the potassium ion selective filtration structure channel in the transmembrane region of KCNJ6 protein, which may result in up regulation of the function of the channel.@*CONCLUSION@#The de novo c.460G>C (p.Gly154Arg) variant of the KCNJ6 gene probably underlay the KPLBS in this child. Above finding has enriched the genotypic and phenotype spectrum of this syndrome.

Expressions of miRNA-146a in peripheral mononuclear cells of children with systemic juvenile idiopathic arthritis and its clinical significance / 中华实用儿科临床杂志

Objective:To investigate the role of miR-146a in the pathogenesis of systemic juvenile idiopathic arthritis (sJIA) and its clinical significance.Methods:This article is a prospective clinical cohort study.Twenty-six patients with sJIA (14 cases of initial active group and 12 cases of stable group), 15 patients with multijoint juvenile idiopathic arthritis (JIA) and 15 patients with oligojoint JIA diagnosed in the Department of Rheumatology and Immunology of Anhui Provincial Children′s Hospital from June 2018 to December 2020 were enrolled.Twenty healthy controls from the out-patient clinic were also recruited.The expression level of miR-146a in peripheral blood mononuclear cells (PBMCs) of research objects was detected by real-time fluorescence quantitative polymerase reaction (qPCR), and the serum levels of interleukin (IL) - 6, tumor necrosis factor (TNF) - α and IL-1β in sJIA patients and healthy controls were detected by enzyme-linked immunosorbent assay.The expression levels of miR-146a in PBMCs and cytokines among different groups were compared by analysis of variance. Pearson correlation analysis was used to analyze the correlation of the relative expression level of miR-146a in PBMCs with clinical inflammatory indexes and serum cytokines in sJIA patients. Results:(1) The expression level of miR-146a in PBMCs of early sJIA patients was significantly higher than that in the multijoint JIA group and oligojoint JIA group (8.77±3.15 vs.4.40±1.59, 2.55±1.15, t=6.27, 14.23; all P<0.05). The expression level of miR-146a in PBMCs of sJIA active patients was significantly higher than that in sJIA stable patients (8.77±3.15 vs.3.63±1.37, t=10.27, P<0.05). There was no significant difference in the expression level of miR-146a between the sJIA stable group and healthy control group ( P>0.05). (2) The expression levels of IL-1β, IL-6 and TNF-α were significantly higher in sJIA active patients group than those in sJIA stable group[(58.56±17.47) ng/L vs.(26.32±10.54) ng/L, (73.72±11.16) ng/L vs.(23.20±9.12) ng/L, (70.93±19.97) ng/L vs.(24.25±9.49) ng/L, all P<0.05]. There was no significant difference in the expression levels of IL-1β, IL-6 and TNF-α between the sJIA stable group and healthy control group(all P>0.05). (3)The expression of miR-146a in PBMCs of sJIA patients was positively correlated with serum ferritin levels, platelets, erythrocyte sedimentation rates, C-reactive proteins, IL-1β, IL-6 and TNF-α( r=0.542, 0.433, 0.329, 0.306, 0.333, 0.342, 0.319, all P<0.05). Conclusions:miR-146a may be involved in the inflammatory process of sJIA disease.miR-146a can well distinguish sJIA from multijoint JIA and oligojoint JIA.TNF-α, IL-1β and IL-6 are involved in sJIA inflammatory responses.

Application Progress of Plant Growth Regulator in Production of Chinese Medicinal Materials / 中国实验方剂学杂志

Plant growth regulator （PGR） is mostly a class of chemical synthesis substance with physiological activities similar to plant hormones，which can promote cell elongation，induce vascular differentiation or accelerate tissue aging via regulating the physiological processes such as photosynthesis，respiration，transpiration，signal transduction，substance absorption and operation. PGR has the advantages of small dosage，high efficiency，low toxicity and less residue，and it is widely used in the planting of Chinese medicinal herbs. By consulting the relevant literature published in recent years，this paper briefly summarizes the main types of PGR，e.g.auxins，gibberellins，cytokinins，abscisic acid and ethylene，etc. On the other hand，this article analyzes and sums up the specific applications of PGR in the manufacture of Chinese herbal medicine，for instance，promoting seed germination，improving seed setting rate or fruit setting rate，dwarfing plants，inhibiting reproductive growth，regulating gender differentiation，stimulating fruit falling，enhancing resistance and so on. The problems existing in the practical use of PGR are pointed out，non-differentiation of specific species，unreasonable combination，not paying attention to the operation method，arbitrarily increasing the dose，lack of residue limit standard and reducing the content of some effective components，for example.Meanwhile，some feasible suggestions are put forward.Not only the suitable types of PGR should be selected in a reasonable and standardized manner，but also the appropriate concentration，dosage and period of application should be chosen carefully； the dual effects of PGR on plant growth and active ingredients in medicinal organs should be concerned，so as to improve the yield and avoid the loss of effective components on the basis of ensuring the quality of Chinese medicinal materials； it is necessary to strengthen the registration of PGR in the production of Chinese medicinal materials and establish residue limit standards to provide a monitoring basis for ensuring the safety of Chinese medicine in the future.The scientific use of PGR can promote the increase of agricultural yield and farmers' income，and make the healthy development of Chinese herbal medicine planting industry.

Diagnostic value and influencing factors of chylous test of pleural effusion after thoracic surgery / 中国胸心血管外科临床杂志

@#Objective    To analyze the results and diagnostic value of postoperative chylous test of pleural effusion and to verify the clinicopathological factors affecting the results of chylous test. Methods    The clinical data of 265 consecutive patients undergoing selective surgery at the Department of Thoracic Surgery, Shangjin Nanfu Hospital between May and August 2020 were retrospectively analyzed, including 106 males and 159 females with an average age of 53.0±12.2 years. According to the results of the chylous test on the operation day and postoperative first and second days, the patients were divided into two subgroups, including a positive group and a negative group, and the clinical data of the two groups were compared. Sensitivity and specificity of the chylous test were calculated. The influencing factors for chylous test were analyzed by multiple logistic regression analysis. Results    The positive rate of chylous test was 91.7%, 95.8% and 87.9% on the operation day and postoperative first and second days, respectively. There was no statistical difference in age, sex, surgical type, surgical approach, surgical site, surgical time, degree of lymph node dissection, treatment of thoracic duct, 24 hours pleural fluid drainage or 24 hours protein and fat food intake between the positive group and the negative group (P>0.05). The diagnostic sensitivity and specificity of the chylous experiment were 100.0%and 4.0%, respectively. Multiple logistic regression analysis showed that the surgical site (left/right chest) might be an influencing factor for the results of the chylous test (P=0.043, OR=0.458, 95%CI 0.216-0.974). Conclusion    The positive rate of chylous test of pleural effusion after thoracic surgery is very high. The chylous test produces a high misdiagnosis rate of chylothorax. The surgical site (left/right chest) may be an influencing factor for chylous test. The positive result of chylous test is not recommended as the direct diagnostic basis for postoperative chylothorax and guidance of the subsequent treatment.

Combination of axitinib with dasatinib improves the outcome of a chronic myeloid leukemia patient with BCR-ABL1 T315I mutation / 中南大学学报(医学版)

Chronic myeloid leukemia (CML) is one of the most common hematological malignancies and characterized by the formation of Philadelphia (Ph) chromosome. Recently, tyrosine kinase inhibitors (TKI) treatment greatly improved the prognosis of CML. However, the options may be limited when a patient develops traditional TKI resistance or gene mutation. Herein, we reported a case. A 38-year-old male CML patient developed a BCR-ABL1 gene mutation of T315I after 2.5 years of TKI treatment, including imatinib and dasatinib. We adjusted the treatment with the combined application of dasatinib and axitinib. BCR-ABL1 gene copies dropped down and achieved an early molecular response at 2 months later. Subsequently, he received hematopoietic stem cell transplantation. Axitinib and dasatinib were applied for another half year after the allogeneic hematopoietic stem cell transplantation (allo-HSCT). Two years after the allo-HSCT, the BCR-ABL1 gene was still undetectable. It provided a successful example in treating CML patients carrying BCR-ABL1 T315I mutation via combination of axitinib with conditional TKI.

Relations between cigarette smoking and chronic diseases of Chinese adults in 2013 / 中华流行病学杂志

Objective To explore the relations between the prevalence of multiple chronic diseases and cigarette smoking behavior in the Chinese adults.Methods Based on the results from the 2013 Chronic Disease Risk Factor Surveillance (NCD Surveillance),176 534 Chinese residents aged 18 years and above,covering 298 counties (districts) in 31 provinces,was randomly recruited,using the multi-stage stratified clustering sampling method.Information on demographics,cigarette smoking (status,quantity and period) was obtained through face-to-face interviews and questionnaires.Anthropometric data and blood samples were collected and properly stored for analysis.Results In total,175 386 adults were included for statistical analyses,with 42.7％ as males and 57.3％ as females.The prevalence rates of hypertension,high total cholesterol and high triglycerides were 30.4％,7.2％ and 18.0％ in male smokers,35.6％,14.0％,10.3％ and 15.9％ in female smokers respectively,which were all higher than those in the respective non-smokers.Male smokers were found under lower risk on hypertension,but 19％ higher on total glycerides when compared with non-smokers of the same sex (OR=1.19,95％CI:1.10-1.30),when multiple risk factors were under control.Male current smokers with more than 20 cigarettes per day have 41％ (OR=1.41,95％CI:1.28-1.55) higher risk of high TG than non-smokers.Female smokers presented 40％ (OR=1.40,95％CI:1.15-1.70) higher risk in high glycerides than the non-smokers.Specifically,women smoking longer than 20 years have 60％(OR=l.60,95％ CI:1.31-1.95) higher risk of high TG than women smoking less than 20 years.Conclusions Prevalence rates of certain chronic diseases were seen higher in smokers of both genders.People with longer history of smoking or being heavier smokers,appeared at advanced risk on developing chronic diseases.

Geographical variation and related factors in prediabetes prevalence in Chinese adults in 2013 / 中华预防医学杂志

Objective@#To investigate the geographical variation of prediabetes in adults in different regions of China, and to analyze the related factors of prediabetes.@*Methods@#Data was obtained from China Chronic Disease and Related Risk Factor Surveillance in 2013. The surveillance adopted multiple-stage stratified cluster random sampling method, which sampled 177 099 residents aged above 18 years old among 298 surveillance points in 31 provinces of Chinese Mainland. Questionnaire interview was used to obtain demographic variables, personal living style, and socio-economical information. Physical examination was conducted and fasting venous blood sample and (oral glucose tolerance test-2 hours, OGTT-2 h) venous blood sample were obtained from the participants. A total of 171 567 residents aged 18 and above were included in the analysis. The prevalence of prediabetes was analyzed by provinces and by China's geographical regions, after complex weighting. Multilevel logistic models were established to explore the related factors of prediabetes on the area level and individual level.@*Results@#The prevalence of prediabetes among residents aged 18 and above was 16.6% (95%CI: 15.6%-17.6%) in China. The prevalence of prediabetes was the highest (18.3%) in the south China and lowest (13.1%) in the northwest area. The difference of the prevalence in different areas were not statistically significant (P=0.510). If categorized the prevalence of prediabetes into 5 groups by quintile, Hainan, Jilin, Shandong, Anhui, Hunan and Chongqing were in the highest group of prevalence of prediabetes (18.6%-22.7%), and Tibet, Qinghai, Gansu, Ningxia, Guizhou, and Jiangxi were in the lowest group (7.6%-12.6%). The variance of prevalence of prediabetes on the county level (MOR: 1.60 (95%CI:1.53-1.67)) was more diverse than the province level (MOR: 1.21(95%CI:1.08-1.29)) and higher than the street level (1.23 (95%CI:1.14-1.30)). Several factors increased risk of pre-diabetes, including smoking, hazardous drinking and harmful drinking, drinking in the past 30 days, overweight, obesity, central obesity, sugary drink intake, hypertension, high total cholesterol, high triglycerides, high blood low-density lipoprotein cholesterol, low blood high-density lipoprotein cholesterol (all P<0.05). After adjusted the above variables, 92.5% of variance of prediabetes prevalence conld be explained on the provincial level.@*Conclusion@#The geographical distribution of prediabetes in adults in China differed by geographic areas, and it significantly varied on the county level. The related variables included demographic variables, personal behavior, and geographic related variables.

Multilevel logistic regression analysis on hypercholesterolemia related risk factors among adults in China / 中华预防医学杂志

Objective@#To investigate the prevalence of hypercholesterolemia among Chinese adults in different geographic areas, and to analyze the related factors.@*Methods@#China Chronic and Non-Communicable Disease and Risk Factor Surveillance was conducted in 2013, based on 298 counties/districts in 31 provinces of Chinese mainland. The adults aged 18 years old were randomly selected using multi-stage stratified clustering sampling method. Information on chronic disease and risk factors was collected using face-to-face questionnaire interview and physical measurement. Blood samples were collected by local staffs. Serum total cholesterol (TC) was determined using standard method in a central laboratory. After excluding 565 participants missing key variables and 1 558 participants with abnormal TC values, a total of 174 976 participants were included. Weighted prevalence of hypercholesterolemia was calculated. Hypercholesterolemia related individual or geographic determinants were defined using multilevel logistic regression.@*Results@#The prevalence of hypercholesterolemia in Chinese adults age 18 years old and above was 6.9% (95%CI: 6.4%-7.3%), ranged from 3.0% (95%CI: 2.5%-3.4%) in the northwest of China to 14.2% (95%CI: 12.9%-15.5%) in the south (χ2=183.42, P<0.001). The prevalence of hypercholesterolemia was higher in 6 provinces including Tianjin, Liaoning, Fujian, Guangdong, Guangxi, and Hainan (≥9.0%), but lower in 7 provinces including Shanxi, Shannxi, Gansu, Qinghai, Ningxia, Xinjiang, and Tibet (<3.9%). There was a 1.27 fold variation in hypercholesterolemia prevalence by provincial level, and 72.5% of the geographical variation in hypercholesterolemia prevalence was account for by area-level determinants. With multilevel logistic analysis, the individual risk factors associated with hypercholesterolemia included aging, higher education level or annual household income per capita, regular drinking, too much red meal intake, inactivity, overweight or obesity. For geographic factors, residents living at south China, counties/districts with higher urbanization rates, higher education level or lower standardized death rates were more likely to have hypercholesterolemia (all P<0.05).@*Conclusion@#The prevalence of hypercholesterolemia was high in Chinese adults, it was different between regions and related with characteristics of population, individual behaviors and geographical regions.

Efficacy and safety of Carbamazepine in treatment of vestibular complications / 中华老年医学杂志

Objective To evaluate the efficacy and safety of Carbamazepin (CBZ)compared with Oxcarbazepine (OXC ) therapy for vestibular paroxysmia. Methods Eighty-two patients with vestibular paroxysmia were admitted during June 2013 and June 2017 in this study. According to the agents administered ,all patients were divided into the CBZ group(n＝ 31) ,CBZ+ Betahistine(BMT) group(n＝ 26)and OXC+ BMT group(n＝ 25).The clinical efficacy ,frequency ,vertigo and adverse reactions of three groups were compared after 3 months follow-up. Results In CBZ group ,14 cases were cured ,13 were improved ,and the effective rate was 87.1%.In CBZ+BMT group ,18 cases were cured ,7 were recovered ,and the effective rate was 96.2%.In OXC+BMT group ,15 cases were cured , 8 cases were recovered ,and the effective rate was 92.0% . There was no significantly difference in effective rate among the three groups(χ2＝0.783 ,P＝0.129).Meanwhile ,the CBZ+BMT group had the lowest frequency of vestibular paroxysmia and vertigo degree ,while the CBZ group was the highest ;the difference in the frequency and vertigo degree between groups was statistically significant (P＜ 0.05).Furthermore ,the incidences of side-effects were 51.6%(n＝ 16) ,30.8%(n ＝ 8)and 16.0%(n＝4)in the CBZ group ,CBZ+BMT group and OXC+BMT group ,respectively. Conclusions The effect of Carbamazepine and Oxcarbazepine for vestibular paroxysmia is similar ,and is safely and significantly improved when combined with Betahistine.

Relations between cigarette smoking and chronic diseases of Chinese adults in 2013 / 中华流行病学杂志

Objective To explore the relations between the prevalence of multiple chronic diseases and cigarette smoking behavior in the Chinese adults.Methods Based on the results from the 2013 Chronic Disease Risk Factor Surveillance (NCD Surveillance),176 534 Chinese residents aged 18 years and above,covering 298 counties (districts) in 31 provinces,was randomly recruited,using the multi-stage stratified clustering sampling method.Information on demographics,cigarette smoking (status,quantity and period) was obtained through face-to-face interviews and questionnaires.Anthropometric data and blood samples were collected and properly stored for analysis.Results In total,175 386 adults were included for statistical analyses,with 42.7％ as males and 57.3％ as females.The prevalence rates of hypertension,high total cholesterol and high triglycerides were 30.4％,7.2％ and 18.0％ in male smokers,35.6％,14.0％,10.3％ and 15.9％ in female smokers respectively,which were all higher than those in the respective non-smokers.Male smokers were found under lower risk on hypertension,but 19％ higher on total glycerides when compared with non-smokers of the same sex (OR=1.19,95％CI:1.10-1.30),when multiple risk factors were under control.Male current smokers with more than 20 cigarettes per day have 41％ (OR=1.41,95％CI:1.28-1.55) higher risk of high TG than non-smokers.Female smokers presented 40％ (OR=1.40,95％CI:1.15-1.70) higher risk in high glycerides than the non-smokers.Specifically,women smoking longer than 20 years have 60％(OR=l.60,95％ CI:1.31-1.95) higher risk of high TG than women smoking less than 20 years.Conclusions Prevalence rates of certain chronic diseases were seen higher in smokers of both genders.People with longer history of smoking or being heavier smokers,appeared at advanced risk on developing chronic diseases.

Web-based early warning management and application of EMRS crossplatform medical insurance expenses / 医疗卫生装备

Objective To integrate the self-developed Web-based early warning management system into EMR system to decrease average medical fee as well as the occurrences of cost overrun,arrearage for self-paid fee and etc.Methods The method mapped into image for users' browse based on using the user-defined URL extension characteristic of EMR system,combining the advantages of Web platform in handling Http request,applying Java as well as SQL technology to obtain source data,integrating statistical analysis for multi-dimensional comparative analysis as well as visualization technology.Results This system changed the behavior pattern of early warning and supervision in course,relieved work burden of medical staffs,and helped hospital achieve high economic management benefit.Conclusion The application of crossplatform system realizes high effect and provides approaches for expanding other functions.

Pim-1 Kinase Regulating Dynamics Related Protein 1 Mediates Sevoflurane Postconditioning-induced Cardioprotection / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>It is well documented that sevoflurane postconditioning (SP) has a significant myocardial protection effect. However, the mechanisms underlying SP are still unclear. In the present study, we investigated the hypothesis that the Pim-1 kinase played a key role in SP-induced cardioprotection by regulating dynamics-related protein 1 (Drp1).</p><p><b>METHODS</b>A Langendorff model was used in this study. Seventy-two rats were randomly assigned into six groups as follows: CON group, ischemia reperfusion (I/R) group, SP group , SP+proto-oncogene serine/threonine-protein kinase 1 (Pim-1) inhibitor II group, SP+dimethylsufoxide group, and Pim-1 inhibitor II group (n = 12, each). Hemodynamic parameters and infarct size were measured to reflect the extent of myocardial I/R injury. The expressions of Pim-1, B-cell leukemia/lymphoma 2 (Bcl-2) and cytochrome C (Cyt C) in cytoplasm and mitochondria, the Drp1 in mitochondria, and the total Drp1 and p-Drp1ser637 were measured by Western blotting. In addition, transmission electron microscope was used to observe mitochondrial morphology. The experiment began in October 2014 and continued until July 2016.</p><p><b>RESULTS</b>SP improved myocardial I/R injury-induced hemodynamic parametric changes, cardiac function, and preserved mitochondrial phenotype and decreased myocardial infarct size (24.49 ± 1.72% in Sev group compared with 41.98 ± 4.37% in I/R group; P< 0.05). However, Pim-1 inhibitor II significantly (P < 0.05) abolished the protective effect of SP. Western blotting analysis demonstrated that, compared with I/R group, the expression of Pim-1 and Bcl-2 in cytoplasm and mitochondria as well as the total p-Drp1ser637 in Sev group (P < 0.05) were upregulated. Meanwhile, SP inhibited Drp1 compartmentalization to the mitochondria followed by a reduction in the release of Cyt C. Pretreatment with Pim-1 inhibitor II significantly (P < 0.05) abolished SP-induced Pim-1/p-Drp1ser637 signaling activation.</p><p><b>CONCLUSIONS</b>These findings suggested that SP could attenuate myocardial ischemia-reperfusion injury by increasing the expression of the Pim-1 kinase. Upregulation of Pim-1 might phosphorylate Drp1 and prevent extensive mitochondrial fission through Drp1 cytosolic sequestration.</p>

Vegetable and Fruit Consumption among Chinese Adults and Associated Factors: A Nationally Representative Study of 170,847 Adults / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>This study examined vegetable and fruit (VF) consumption rate and its associated factors among Chinese adults.</p><p><b>METHODS</b>Nationally representative data from the 2013 China Chronic Disease Surveillance survey were used. Dietary intake data, including VF consumption during the last 12 months, were collected. All analyses were weighted to obtain nationally representative estimates. Associations between VF consumption and other factors (e.g., meal frequency and physical activity) were examined through logistic regression analysis.</p><p><b>RESULTS</b>The average fruit consumption was 102.3 g/day (95% CI: 97.0-107.6) and the average vegetable consumption was 350.6 g/day (95% CI: 339.3-361.8). Over half (53.2%, 95% CI: 50.9-55.4) of Chinese adults met the VF consumption of 400 g/day recommended by the World Health Organization (WHO). Rural residents had a higher prevalence of low VF consumption rate than urban residents [49.20% (95% CI: 46.2%-52.2%) vs. 44.0% (95% CI: 41.7%-46.3%) P < 0.01]. Old age (OR = 1.01, 95% CI: 1.00-1.01), low educational level, low income, minority ethnicity (OR = 1.41, 95% CI: 1.15-1.74), underweight (OR = 1.17, 95% CI: 1.03-1.33), single marital status (OR = 1.20, 95% CI: 1.08-1.33), low health literacy, irregular breakfast (OR = 1.20, 95% CI: 1.04-1.38) or lunch (OR = 1.58, 95% CI: 1.26-1.99) habits, and no leisure-time physical activity were associated with low VF consumption.</p><p><b>CONCLUSION</b>Only half of Chinese adults met the VF consumption recommended by the WHO. Low socio-economic status, irregular diet, and poor health literacy were likely associated with low VF consumption. National efforts and programs are needed to promote VF consumption.</p>

Management of diabetes patients aged ≥35 years in disease surveillance areas in China / 中华流行病学杂志

Objective To investigate the community-based management of diabetes patients aged ≥35 years in China.Methods The subjects from 2013-2014 Chronic Non-communicable Disease and Risk Factor Surveillance in China were used in this study,those who were aged ≥35 years and diagnosed by doctors in hospitals at community level or above were selected through clustering sampling.Questionnaire was used to collect the data of subjects' general information,health status,the treatment and the control of blood glucose.Blood samples were taken from the subjects to detect the fasting blood glucose level and blood glucose level at 2 hours after oral administration of glucosum anhydricum.The subjects were weighted according to complex sampling scheme to calculated the different rates and 95％CI.The Rao-scott x2 test was performed to test the differences in rates between the subgroups.Results The survey indicated that among the 10 056 diabetes patients aged ≥35 years and diagnosed with diabetes,4 609 received management service in communities.After being weighted,the management rate of diabetes patients was 45.0％ (95％ CI:40.8％-49.2％).Females (46.9％,95％CI:42.8％-51.0％) had higher management rate than males (43.0％,95％CI:38.1％-47.9％).The management rate was higher in rural area (50.4％,95％CI:46.3％-54.5％) than in urban area (41.6％,95％CI:35.5％-47.6％).There was a significant age specific difference in the proportion of patients receiving management services (x2=21.0,P＜0.01),the rate of management was highest in the patients aged ≥65 years (49.2％,95％CI:43.6％-54.7％),but lowest in the patients aged 35-44 years (35.2％,95％CI:27.9％-42.4％).The overall standardized management rate of diabetes patients in communities was 16.7％ (95％ CI:13.7％-19.7％).The proportion of urban patients receiving standardized management service (19.7％,95％CI:15.3％-24.1％) was higher than that of rural patients (12.8％,95％ CI:9.8％-15.8％).The overall treatment rate of diabetes patients in communities was 95.8％ (95％CI:94.8％-96.9％).The treatment rate was higher in females (97.0％,95％CI:96.0％-98.0％) than that in males (94.5％,95％CI:92.7％-96.4％).The control rate of blood glucose in diabetes patients receiving management in communities was 34.6％ (95％CI:31.5％-37.6％),and the highest blood glucose control rate was in the patients aged ≥65 years (38.2％,95％ CI:33.4％-43.0％),while the lowest blood glucose control rate was in the patients aged 45-54 years (34.4％,95％CI:26.7％-42.0％).Conclusions Both the standardized management rate and blood glucose control rate were low in the diabetes patients aged ≥35 years in China.It is necessary to strengthen the allocation of medical resources in communities and standardized diabetes management.

Quality of life and related influencing factors in Chinese adults / 中华流行病学杂志

Objective To evaluate the quality of life (QOL) and influencing factors on Chinese adults.Methods 83 666 subjects from 2010 Chronic Non-communicable Disease and Risk Factor Surveillance Project in China were included in this study.Questionnaire was used to collect information on general condition and health status.WHOQOL-BREF was adopted as an instrument to measure the QOL on all the subjects,t test was used to compare QOL from different groups.Multiple linear regression analysis was used to assess the association of QOL with BMI,by gender.Results Among all the 83 666 subjects,mean scores of physical,psychological,social relationship and environment domains appeared as (73.97 ± 13.84),(66.65 ± 14.21),(65.76 ± 14.08) and (56.59 ± 15.15),respectively.Age,residential areas (urban/rural),education levels and marital status all showed significant impact on scores of the four domains (P＜0.05).Scores decreased with age and increased with education level (P＜0.05).Scores of rural adults were greater than those from the urban and the scores of married or cohabiting adults were greater than the other groups (P＜0.05).Physical and psychological scores were lower in women than that of in men (P＜0.05).Except for environment domain,scores of patients with chronic diseases were much lower than those without.Conclusion Factors as age,residence (urban/rural),education levels,marital status and chronic diseases could significantly influence the QOL of Chinese adults.

P38 MAPK signaling pathway mediates advanced oxidation protein product-induced epithelial-to-mesenchymal transition in tubular cells / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate whether the p38 mitogen-activated protein kinase (MAPK) signaling pathway mediates advanced oxidation protein products (AOPPs)-induced epithelial-to-mesenchymal transition (EMT) in tubular cells.</p><p><b>METHODS</b>Human proximal tubular cells (HK-2 cells) exposed to AOPP-bovine serum albumin (BSA) were examined for expressions of p38 MAPK and phosphorylated p38 MAPK using Western blotting. Western blotting and quantitative RT-PCR were used to examine the protein and mRNA expressions of EMT markers E-cadherin and vimentin and endoplasmic reticulum stress marker glucose-regulated protein (GRP) 78 in cells treated with SB203580 (an inhibitor of the p38 MAPK signaling pathway) prior to AOPP exposure. The cells treated with AOPPs following pretreatment with salubrinal (an inhibitor of endoplasmic reticulum stress) were also examined for expressions of p38 MAPK and phosphorylated p38 MAPK.</p><p><b>RESULTS</b>AOPP treatment induced the phosphorylation of p38 MAPK in HK-2 cells. AOPP-induced decrease in E-cadherin expression and overexpression of vimentin and GRP78 were partly inhibited by pretreatment of the cells with SB203580. Salubrina partly suppressed AOPP-induced phosphorylation of p38 MAPK in the cells.</p><p><b>CONCLUSION</b>p38 MAPK signaling pathway, which is regulated by endoplasmic reticulum stress, might mediate AOPP-induced EMT in HK-2 cells.</p>

Management of diabetes patients aged ≥35 years in disease surveillance areas in China / 中华流行病学杂志

Objective To investigate the community-based management of diabetes patients aged ≥35 years in China.Methods The subjects from 2013-2014 Chronic Non-communicable Disease and Risk Factor Surveillance in China were used in this study,those who were aged ≥35 years and diagnosed by doctors in hospitals at community level or above were selected through clustering sampling.Questionnaire was used to collect the data of subjects' general information,health status,the treatment and the control of blood glucose.Blood samples were taken from the subjects to detect the fasting blood glucose level and blood glucose level at 2 hours after oral administration of glucosum anhydricum.The subjects were weighted according to complex sampling scheme to calculated the different rates and 95％CI.The Rao-scott x2 test was performed to test the differences in rates between the subgroups.Results The survey indicated that among the 10 056 diabetes patients aged ≥35 years and diagnosed with diabetes,4 609 received management service in communities.After being weighted,the management rate of diabetes patients was 45.0％ (95％ CI:40.8％-49.2％).Females (46.9％,95％CI:42.8％-51.0％) had higher management rate than males (43.0％,95％CI:38.1％-47.9％).The management rate was higher in rural area (50.4％,95％CI:46.3％-54.5％) than in urban area (41.6％,95％CI:35.5％-47.6％).There was a significant age specific difference in the proportion of patients receiving management services (x2=21.0,P＜0.01),the rate of management was highest in the patients aged ≥65 years (49.2％,95％CI:43.6％-54.7％),but lowest in the patients aged 35-44 years (35.2％,95％CI:27.9％-42.4％).The overall standardized management rate of diabetes patients in communities was 16.7％ (95％ CI:13.7％-19.7％).The proportion of urban patients receiving standardized management service (19.7％,95％CI:15.3％-24.1％) was higher than that of rural patients (12.8％,95％ CI:9.8％-15.8％).The overall treatment rate of diabetes patients in communities was 95.8％ (95％CI:94.8％-96.9％).The treatment rate was higher in females (97.0％,95％CI:96.0％-98.0％) than that in males (94.5％,95％CI:92.7％-96.4％).The control rate of blood glucose in diabetes patients receiving management in communities was 34.6％ (95％CI:31.5％-37.6％),and the highest blood glucose control rate was in the patients aged ≥65 years (38.2％,95％ CI:33.4％-43.0％),while the lowest blood glucose control rate was in the patients aged 45-54 years (34.4％,95％CI:26.7％-42.0％).Conclusions Both the standardized management rate and blood glucose control rate were low in the diabetes patients aged ≥35 years in China.It is necessary to strengthen the allocation of medical resources in communities and standardized diabetes management.

Quality of life and related influencing factors in Chinese adults / 中华流行病学杂志

Objective To evaluate the quality of life (QOL) and influencing factors on Chinese adults.Methods 83 666 subjects from 2010 Chronic Non-communicable Disease and Risk Factor Surveillance Project in China were included in this study.Questionnaire was used to collect information on general condition and health status.WHOQOL-BREF was adopted as an instrument to measure the QOL on all the subjects,t test was used to compare QOL from different groups.Multiple linear regression analysis was used to assess the association of QOL with BMI,by gender.Results Among all the 83 666 subjects,mean scores of physical,psychological,social relationship and environment domains appeared as (73.97 ± 13.84),(66.65 ± 14.21),(65.76 ± 14.08) and (56.59 ± 15.15),respectively.Age,residential areas (urban/rural),education levels and marital status all showed significant impact on scores of the four domains (P＜0.05).Scores decreased with age and increased with education level (P＜0.05).Scores of rural adults were greater than those from the urban and the scores of married or cohabiting adults were greater than the other groups (P＜0.05).Physical and psychological scores were lower in women than that of in men (P＜0.05).Except for environment domain,scores of patients with chronic diseases were much lower than those without.Conclusion Factors as age,residence (urban/rural),education levels,marital status and chronic diseases could significantly influence the QOL of Chinese adults.